Synchrotron X-ray Science

Figure 1. Experimental hall at the NSLS-II.

Synchrotron X-ray sources are powerful tools for learning about nanoscale structure and dynamics. These particle accelerators create extremely bright X-rays that enable new kinds of experiments. I apply synchrotron X-rays to problems in biophysics and conbribute to the development of new methods. These methods include cryo-SAXS, anomalous SAXS, time-resolved SAXS, X-ray diffuse scattering, and chromatography-coupled SAXS (see Further Reading, below).

I collect data at several synchrotron X-ray facilities, including the Cornell High Energy Synchrotron Source (CHESS), the Advanced Photon Source (APS), and the National Synchrotron Light Source II (NSLS-II).

I am active in the synchrotron user community, and have served on the CHESS User Executive Committee since 2016.

Here is a list of beamlines (experimental end stations) I’ve used:

Facility	Beamline	Technique	Publications*
CHESS	G1	solution scattering	17 articles
	F1	crystallography, diffuse scattering	2 articles
	C1	anomalous scattering	6 articles
APS	BioCAT	solution scattering	1 article
	NECAT	crystallography	(none yet)
NSLS-II	LiX	solution scattering	(none yet)

*Links on the right point to my publications involving data from each beamline.

Steve P. Meisburger

Synchrotron X-ray Science

Related Articles

Related Articles

Pabit SA, Meisburger SP, Li L, Blose JM, Jones CD, Pollack L. (2010). Counting ions around DNA with anomalous small-angle X-ray scattering. JACS 132(46): 16334-6.

Meisburger SP, Warkentin M, Chen H, Hopkins JB, Gillilan RE, Pollack L, Thorne RE. (2013). Breaking the radiation damage limit with Cryo-SAXS. Biophys. J. 104(1): 227-36.

Meisburger SP, Warkentin MA, Hopkins JB, Katz AM, Pollack L, and Thorne RE. (2013). Apparatus and methods for low temperature small angle x-ray scattering. Patent US: 9927336.

Chen Y, Tokuda JM, Topping T, Sutton JL, Meisburger SP, Pabit SA, Gloss LM, Pollack L. (2014). Revealing transient structures of nucleosomes as DNA unwinds. Nucl. Acids Res. 42: 8767-76.

Nguyen HT, Pabit SA, Meisburger SP, Pollack L, Case DA. (2014). Accurate small and wide angle x-ray scattering profiles from atomic models of proteins and nucleic acids. J. Chem. Phys. 141(22): 22D508.

Hopkins JB, Katz AM, Meisburger SP, Warkentin MA, Thorne RE, Pollack L. (2015). A microfabricated fixed path length silicon sample holder improves background subtraction for cryoSAXS. J. Appl. Cryst. 48: 227–37.

Meisburger SP, Pabit SA, Pollack L. (2015). Determining the locations of ions and water around DNA from X-ray scattering measurements. Biophys. J. 108(12): 2886-95.

Chen Y, Tokuda JM, Topping T, Meisburger SP, Pabit SA, Gloss LM, Pollack L. (2016). Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core. PNAS 114: 334-9.

Meisburger SP, Taylor AB, Khan CA, Zhang S, Fitzpatrick PF, Ando N. (2016). Domain movements upon activation of phenylalanine hydroxylase characterized by crystallography and chromatography-coupled small-angle X-ray scattering. JACS 138(20): 6506–16.

Meisburger SP, Ando N. (2017). Correlated Motions from Crystallography beyond Diffraction. Acc. Chem. Res. 50(3): 580–3.

Meisburger SP^†, Thomas WC^†, Watkins MB^†, Ando N. (2017). X-ray scattering studies of protein structural dynamics. Chem. Rev. 117: 7615–72.

Parker MJ, Maggiolo AO, Thomas WC, Kim A, Meisburger SP, Ando N, Boal AK, Stubbe J. (2018). An endogenous dAMP ligand in Bacillus subtilis class Ib RNR promotes assembly of a noncanonical dimer for regulation by dATP. PNAS 115(20): E4594-E4603.

Khan CA, Meisburger SP, Ando N, Fitzpatrick PF. (2019). The phenylketonuria-associated substitution R68S converts phenylalanine hydroxylase to a constitutively active enzyme but reduces its stability. JBC 294(12): 4359-67.

Meisburger SP, Case DA, Ando N. (2020). Diffuse X-ray scattering from correlated motions in a protein crystal. Nature Communications 11: 1271.

Meisburger SP^†, Xu D^†, Ando N. (2021). REGALS: a general method to deconvolve X-ray scattering data from evolving mixtures. IUCrJ 8(2): 225-237.

Xu D^†, Meisburger SP^†, Ando N. (2021). Correlated motions in structural biology. Biochemistry 60: 2331–2340.